nih 3t3 fibroblasts  (ATCC)

Journal: Bioactive Materials

Article Title: Slippery dopamine–fluoropolymer hybrid surface for improving biliary stent longevity

doi: 10.1016/j.bioactmat.2026.02.003

Figure Lengend Snippet: In vitro cell evaluations. (a, b) Fluorescence microscopic images of NIH 3T3 cells stained with a live/dead kit and corresponding quantitative analysis (n = 4) (scale bars, 100 μm). (c) Cytotoxicity analysis with NIT-3T3 cells using CCK-8 kit (n = 4). (d, e) Morphological analysis of NIH 3T3 cells stained for actin (red) and nucleus (blue), with fibroblast aspect ratio analysis (scale bars, 100 μm) (n = 4). (f) Schematic illustration demonstrating the selective application of ELFS coating to the target region. (g, h) Fluorescence images showing selective adhesion of NIH 3T3 and RAW 264.7 cells to ELFS-uncoated region (n = 4) (scale bars, 100 μm). (i, j) Optical images and quantification of adhered colony-forming units (CFUs) on non-coated and ELFS-coated plates after incubation in E. coli and S. aureus suspensions for 24 h (n = 4). (k) Sequential SEM images depicting biofilm formation on non-coated and ELFS- coated stent fragments (n = 3) (scale bars, 0.5 μm). (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001). ns, not significant.

Article Snippet: The prepared stents were placed on the Transwell insert, and NIH-3T3 fibroblasts (ATCC CRL-1658; 0.5 × 10 5 cells mL −1 ) or human biliary epithelial SNU-1079 cells (Korean Cell Line Bank, KCLB No. 01079; 0.5 × 10 5 cells mL −1 ) were cultured in 2 mL of DMEM supplemented with 10% bovine calf serum and 1% penicillin–streptomycin.

Techniques: In Vitro, Fluorescence, Staining, CCK-8 Assay, Incubation

Journal: Materials Today Bio

Article Title: Crosslinked hyaluronic acid-doped polypyrrole: Stable, nonbiofouling implantable bioelectrodes for in vivo signal recording

doi: 10.1016/j.mtbio.2026.103182

Figure Lengend Snippet: Surface characterization of PPy/HA and PPy/cHA after hyaluronidase (HAase) treatment. (a) WCAs of gold, PPy/HA, and PPy/cHA before and after HAase treatment. (b) Quantification of surface carboxyl groups on PPy/HA and PPy/cHA after HAase treatment. (c) Electrochemical impedance spectra of gold, PPy/HA, and PPy/cHA electrodes with or without HAase treatment. (d) Relative impedance changes (%) at 1 Hz for PPy/HA and PPy/cHA electrodes after HAase treatment. The impedance value at 1 Hz of each electrode was normalized to that of HAase non-treated PPy/HA. (e) Representative optical micrographs of NIH-3T3 fibroblasts adhered on gold, PPy/HA, and PPy/cHA with and without HAase treatment. Scale bar = 200 μm. (f) Quantification of adhered cell numbers. An asterisk (∗) denotes a statistically significant difference (p < 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Mouse NIH-3T3 fibroblasts (Korean Cell Line Bank, Seoul, Republic of Korea) were seeded on each sample to evaluate cell adhesion.

Techniques:

Journal: Materials Today Bio

Article Title: Crosslinked hyaluronic acid-doped polypyrrole: Stable, nonbiofouling implantable bioelectrodes for in vivo signal recording

doi: 10.1016/j.mtbio.2026.103182

Figure Lengend Snippet: In vitro cytotoxicity tests. (a) Representative live/dead fluorescence images of NIH-3T3 fibroblasts cultured in extract media obtained from gold, PPy/HA, and PPy/cHA electrodes after 24 h of incubation. Live and dead cells are stained green and red, respectively. Scale bars = 200 μm. (b) Live cell percentages (cell viability). (c) Metabolic activity of cells assessed by WST-1 assay after 24 h incubation with extract media from gold, PPy/HA, and PPy/cHA electrodes. An asterisk (∗) denotes a statistically significant difference (p < 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Mouse NIH-3T3 fibroblasts (Korean Cell Line Bank, Seoul, Republic of Korea) were seeded on each sample to evaluate cell adhesion.

Techniques: In Vitro, Fluorescence, Cell Culture, Incubation, Staining, Activity Assay, WST-1 Assay

Journal: Frontiers in Pharmacology

Article Title: Extracellular phosphatidylcholine functions as a noncanonical TLR4 activator to drive adipocyte lipolysis and apoptosis

doi: 10.3389/fphar.2026.1783264

Figure Lengend Snippet: Effects of PC and TLR4 inhibition on adipocyte viability, lipolysis, and lipid accumulation. (A) The viability of differentiated 3T3-L1 adipocytes was assessed via a WST assay. Initially, a dose-response analysis was performed by treating cells with various concentrations of PC (0–25 mg/mL) to establish the cytotoxic profile (left panel). Subsequently, cell viability was evaluated following treatment with PC (25 mg/mL) in the presence or absence of the TLR4 inhibitor TAK-242 (1 or 10 nM) (right panel). PC treatment significantly reduced cell viability in a concentration-dependent manner, which was reversed by TAK-242 in a dose-dependent manner, indicating specific receptor-mediated cytotoxicity (n = 16). (B) Glycerol release, a direct indicator of lipolytic activity, was measured in the culture supernatant (absorbance at 570 nm). PC-induced glycerol release was significantly suppressed by TAK-242 pretreatment, confirming the presence of TLR4-dependent lipolysis (n = 16). (C) Intracellular lipid accumulation was quantified using Oil Red O (ORO) staining (absorbance at 492 nm). PC treatment resulted in a substantial reduction in lipid content, which was dose-dependently restored by TLR4 inhibition (n = 8). The data are presented as the mean ± SEM. *p < 0.05, **p < 0.01 vs. the indicated groups.

Article Snippet: Murine 3T3-L1 fibroblasts were obtained from the American Type Culture Collection (ATCC; Manassas, VA, United States).

Techniques: Inhibition, WST Assay, Concentration Assay, Activity Assay, Staining

Journal: Frontiers in Pharmacology

Article Title: Extracellular phosphatidylcholine functions as a noncanonical TLR4 activator to drive adipocyte lipolysis and apoptosis

doi: 10.3389/fphar.2026.1783264

Figure Lengend Snippet: PC-induced adipocyte apoptosis is mediated via TLR4 signaling. Representative images and quantitative analysis showing the pro-apoptotic effects of PC on 3T3-L1 adipocytes (n = 3). (A) Annexin V (green) and DAPI (blue) fluorescence staining (top) and the quantitative analysis of the number of apoptotic cells per unit area (bottom). PC (25 mg/mL) treatment significantly increased the apoptotic cell population, an effect that was dose-dependently and significantly reversed by the TLR4 inhibitor TAK-242 (1 or 10 nM). (B) Representative Western blot bands for procaspase-3 and cleaved caspase-3 (top) and the corresponding densitometric analysis of cleaved caspase-3 (bottom). PC-induced activation of the executioner caspase-3 was effectively suppressed by TLR4 inhibition, confirming that PC triggers receptor-mediated programmed cell death. The data are presented as the mean ± SEM. *p < 0.05 vs. the PC-treated group.

Article Snippet: Murine 3T3-L1 fibroblasts were obtained from the American Type Culture Collection (ATCC; Manassas, VA, United States).

Techniques: Fluorescence, Staining, Western Blot, Activation Assay, Inhibition

Journal: Frontiers in Pharmacology

Article Title: Extracellular phosphatidylcholine functions as a noncanonical TLR4 activator to drive adipocyte lipolysis and apoptosis

doi: 10.3389/fphar.2026.1783264

Figure Lengend Snippet: PC functionally activates the TLR4-NF-κB-TNF-α signaling axis. Representative Western blot images and densitometric analysis showing the activation of downstream inflammatory signaling in differentiated 3T3-L1 adipocytes (n = 3). (A) Expression levels of the pro-inflammatory cytokine TNF-α. PC treatment markedly upregulated TNF-α expression, which was significantly attenuated by TAK-242 in a dose-dependent manner. (B) Functional activation of the NF-κB pathway assessed by the phosphorylation of NF-κB p65 (p-NF-κB). PC treatment triggered a robust increase in p-NF-κB levels, providing definitive proof of signaling activation; this effect was effectively blocked by TAK-242. (C) Total protein expression of TLR4. Despite the robust activation of downstream markers, total TLR4 abundance remained unchanged across all experimental groups, suggesting that PC-mediated signaling involves functional activation of the receptor rather than changes in its protein stability. β-actin was used as a loading control. The data are presented as the mean ± SEM. *p 0.05, **p < 0.01 vs. the PC-treated group.

Article Snippet: Murine 3T3-L1 fibroblasts were obtained from the American Type Culture Collection (ATCC; Manassas, VA, United States).

Techniques: Western Blot, Activation Assay, Expressing, Functional Assay, Phospho-proteomics, Control

Journal: Plants

Article Title: Phytochemistry and Wound-Healing, Enzyme-Inhibitory, and Antifungal Activities of the Wild Forage Legume Lotus rectus L.

doi: 10.3390/plants15091367

Figure Lengend Snippet: Effect of the aqueous leaf extract of L. rectus (LRAE) on cell viability of ( A ) HaCaT keratinocytes and ( B ) NIH/3T3 fibroblasts through resazurin assay. The results are expressed as a percentage of cell viability compared to the control. Columns and bars represent mean and standard deviation, respectively ( n = 3 independent experiments performed in duplicate). Statistical analysis was performed by one-way ANOVA followed by Dunnett’s Multiple Comparison Test: * p < 0.05, compared to the control.

Article Snippet: Human keratinocytes (HaCaT cell line, CLS Cell Lines Service GmbH, Cytion, Eppelheim, Germany), originally described by Boukamp et al. [ ], and murine fibroblasts (NIH/3T3, ATCC CRL-1658, Manassas, VA, USA) were maintained in Dulbecco’s Modified Eagle Medium (DMEM; 41965-039, Gibco, Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% ( v / v ) heat-inactivated fetal bovine serum (FBS; A5256701, Gibco) and 1% ( v / v ) penicillin–streptomycin (15070-063, Gibco).

Techniques: Resazurin Assay, Control, Standard Deviation, Comparison

Journal: Plants

Article Title: Phytochemistry and Wound-Healing, Enzyme-Inhibitory, and Antifungal Activities of the Wild Forage Legume Lotus rectus L.

doi: 10.3390/plants15091367

Figure Lengend Snippet: Evaluation of the wound healing effect of the aqueous leaf extract of Lotus rectus L. (LRAE) on NIH/3T3 fibroblasts (Scratch assay). Data are expressed as a comparison, as a percentage of wound closure relative to control, between the wound area at 0 and 18 hours. Columns and bars represent mean and standard deviation, respectively ( n = 3 independent experiments performed in duplicate). Statistical analysis was performed by one-way ANOVA followed by Dunnett’s Multiple Comparison Test: * p < 0.05, ** p < 0.01, compared to control. Magnification = 10×; scale bar = 10 µm.

Article Snippet: Human keratinocytes (HaCaT cell line, CLS Cell Lines Service GmbH, Cytion, Eppelheim, Germany), originally described by Boukamp et al. [ ], and murine fibroblasts (NIH/3T3, ATCC CRL-1658, Manassas, VA, USA) were maintained in Dulbecco’s Modified Eagle Medium (DMEM; 41965-039, Gibco, Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% ( v / v ) heat-inactivated fetal bovine serum (FBS; A5256701, Gibco) and 1% ( v / v ) penicillin–streptomycin (15070-063, Gibco).

Techniques: Wound Healing Assay, Comparison, Control, Standard Deviation

Journal: MicrobiologyOpen

Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

doi: 10.1002/mbo3.70270

Figure Lengend Snippet: Effect of 10 Gy X‐ray on viability of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells. Blue: untreated controls; Pink: irradiated cells. Cell counts were conducted at 24, 48, and 72 h post‐treatment. Statistical analysis was performed using two‐way ANOVA with Tukey's post hoc test: p < 0.05 (*), p < 0.005 (**), p < 0.0005 (***), p < 0.0001 (****), n = 3. Note: Y ‐axes are scaled independently for each cell line to account for differences in baseline growth, allowing clearer visualization of treatment effects. Tumor cell counts and representative images can be found in Appendix Table and Figure .

Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

Techniques: Irradiation

Journal: MicrobiologyOpen

Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

doi: 10.1002/mbo3.70270

Figure Lengend Snippet: Images of untreated NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells and cells exposed to X‐ray. Scalebar: 300 µm.

Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

Techniques:

Journal: MicrobiologyOpen

Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

doi: 10.1002/mbo3.70270

Figure Lengend Snippet: Viability of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells after treatment with IMP‐1700 (5 µM), ciprofloxacin (CPX) (15 µM), their combination, or DMSO, with and without 10 Gy X‐ray. Cell counts were measured at 24, 48, and 72 h post‐treatment. Statistical significance assessed via two‐way ANOVA and Tukey's post hoc test: p < 0.05 (*), p < 0.005 (**), p < 0.0005 (***), p < 0.0001 (****), n = 3. Significant differences are only shown compared to untreated or 10 Gy. Tumor cell count values and images are available in Appendix Table and Figure , , , .

Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

Techniques: Cell Characterization

Journal: MicrobiologyOpen

Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

doi: 10.1002/mbo3.70270

Figure Lengend Snippet: Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with IMP‐1700, with and without X‐ray. Scalebar: 300 µm.

Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

Techniques:

Journal: MicrobiologyOpen

Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

doi: 10.1002/mbo3.70270

Figure Lengend Snippet: Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with ciprofloxacin (CPX), with and without X‐ray. Scalebar: 300 µm.

Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

Techniques:

Journal: MicrobiologyOpen

Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

doi: 10.1002/mbo3.70270

Figure Lengend Snippet: Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with IMP‐1700 and ciprofloxacin (CPX), with and without X‐ray. Scalebar: 300 µm.

Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

Techniques:

Journal: MicrobiologyOpen

Article Title: Synergistic Effects of IMP‐1700, Ciprofloxacin, and X‐Ray Radiation in Bacteria and Mammalian Cell Lines: Implications for Use in Antimicrobial‐Resistant Bacteria

doi: 10.1002/mbo3.70270

Figure Lengend Snippet: Images of NIH‐3T3, B16.F10, MDA‐MB‐231, and HepG2 cells treated with DMSO, with and without X‐ray. Scalebar: 300 µm.

Article Snippet: The tumor cell lines, mouse melanoma B16.F10 (CRL‐6475, ATCC), human breast cancer MB‐MDA‐231 (CRM‐HTB‐26, ATCC), human hepatocellular carcinoma HepG2 C3A (a derivative of HepG2, CRL‐3581, ATCC), as well as the noncancerous mouse fibroblast cell line NIH‐3T3 (CRL‐1658, ATCC), were cultivated at 37°C, 5% CO2.

Techniques: